Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing <i>Anthocleista nobilis</i>

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td class="align_left">Condition</td><td class="align_center">Value/specification</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left">Column</td><td class="align_center">Supelcosil C18 (150 × 4.6 mm, 3 <i>μ</i>m)</td></tr><tr><td class="align_left">Flow rate</td><td class="align_center">0.8 mL/min</td></tr><tr><td class="align_left">Wavelength of detection</td><td class="align_center">274 nm</td></tr><tr><td class="align_left">Injection volume</td><td class="align_center">50 <i>μ</i>L</td></tr><tr><td class="align_left">Temperature</td><td class="align_center">28°C</td></tr><tr><td class="align_left">Mobile phase</td><td class="align_center">Methanol : 0.1% formic acid (65 : 35, v/v)</td></tr><tr><td class="align_left">Retention time (mean ± SD)</td><td class="align_center">2.374 ± 0.0065 min (<i>n</i> = 11)</td></tr><tr><td class="align_left">Mode of elution</td><td class="align_center">Isocratic</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>HPLC conditions for analysis of brucine.</div>

International Journal of Analytical Chemistry

tab3

Table 3

Table 3: Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing <i>Anthocleista nobilis</i> 

International Journal of Analytical Chemistry

Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

Table 3