Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

Table 4

Percentage recovery (accuracy) of brucine in product HBS1.

Brucine content in product (mg/mL); A	Amount of brucine used to spike product (mg/mL); B	Mean brucine content in spiked product (mg/mL); C	Amount recovered (mg/mL); C − A	Mean % recovery ± SD; (C − A)/B × 100%
0.00895	0.08	0.08965	0.0807	100.90 ± 0.07
0.00895	0.10	0.10915	0.1002	100.24 ± 0.07
0.00895	0.12	0.12995	0.1210	100.81 ± 0.07

All results are means of triplicate determinations. Acceptance criteria for percentage recovery: 100 ± 15% [12].