Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

Table 6

Robustness of the developed method.

Sample	Temperature (°C)	Mean peak area ± SD	RSD (%)	Wavelength (nm)	Mean peak area ± SD	RSD (%)
Brucine	23	16806 ± 8.19	0.05	273	17373 ± 13.06	0.08
	28	16981 ± 98.43	0.58	274	16908 ± 46.82	0.28
	33	16733 ± 6.56	0.04	275	16180 ± 15.38	0.10

All results are means of triplicate determinations. Acceptance criteria for RSD ≤2 [11].