Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

Table 7

Content of brucine in extracts and market samples analyzed.

Sample	Mean peak area ± SD	Mean % content (×10^–3% w/v) ± SD	Extract	Mean peak area ± SD	Mean % content (% w/v) ± SD
HBS1	699.07 ± 0.78	0.8950 ± 0.0046	Water	4573.66 ± 9.04	0.0324 ± 0.00054
HBS2	1564.60 ± 2.95	1.4050 ± 0.0174	96% ethanol	5378.05 ± 88.54	0.1259 ± 0.01798
HBS3	1461.15 ± 4.56	1.3440 ± 0.0269	Ethyl acetate	1501.88 ± 2.20	0.0507 ± 0.00048
HBS4	3425.29 ± 14.45	2.5013 ± 0.0852	70% ethanol	4530.34 ± 6.55	0.0177 ± 0.00022
HBS5	2735.73 ± 1.89	2.0951 ± 0.0112

All results are means of triplicate determinations.