Rapid Separation of Asiatic Acid, Quercetin, and Kaempferol from Traditional Chinese Medicine Centella asiatica (L.) Urban Using HSCCC-Semi-Prep-HPLC and the Assessment of Their Potential as Fatty Acid Synthase Inhibitors

Table 3

Distribution coefficient of AA in 10 different solvent systems.

Serial number	Solvent system	Component ratio (V : V : V : V)	K value
1	A-1	1 : 1 : 1 : 1	0.49
2	A-2	1 : 1.1 : 1 : 1	0.61
3	A-3	1 : 1.1 : 0.9 : 1	0.96
4	A-4	1.5 : 1 : 0.9 : 1	0.75
5	A-5	1.5 : 1 : 0.8 : 1	0.11
6	B-1	1.1 : 1 : 1.1 : 1	0.94
7	B-2	1.5 : 1 : 0.8 : 1	1.01
8	C-1	1 : 1 : 1 : 1	0.25
9	C-2	1.5 : 1 : 0.9 : 1	0.43
10	C-3	1.8 : 1 : 0.8 : 1	0.27

Solvent system A: n-hexane-n-butanol-methanol-water. Solvent system B: n-hexane-n-butanol-ethanol-water. Solvent system C: n-hexane-n-butanol-acetonitrile-water.