Research Article
Fast and Sensitive Detection of SARS-CoV-2 Nucleic Acid Using a Rapid Detection System Free of RNA Extraction
Table 3
LoD determination study results.
| | Titer SARS-CoV-2 virus (copies/mL) | Replicates | ORF1ab gene positive result (%) | N gene positive result (%) | Percentage of positive rate (%) |
| | 2000 | 4 | 100 (4/4) | 100 (4/4) | 100 (4/4) | | 1000 | 4 | 100 (4/4) | 100 (4/4) | 100 (4/4) | | 500 | 4 | 100 (4/4) | 100 (4/4) | 100 (4/4) | | 400 | 4 | 100 (4/4) | 100 (4/4) | 100 (4/4) | | 300 | 4 | 100 (4/4) | 100 (4/4) | 100 (4/4) | | 200 | 4 | 75 (3/4) | 75 (3/4) | 75 (3/4) | | 300 | 20 | 100 (20/20) | 100 (20/20) | 100 (20/20) | | 250 | 20 | 95 (19/20) | 90 (18/20) | 95 (19/20) | | 200 | 20 | 80 (16/20) | 85 (17/20) | 95 (19/20) | | 150 | 20 | 80 (16/20) | 80 (16/20) | 90 (18/20) | | 100 | 20 | 50 (10/20) | 60 (12/20) | 70 (14/20) |
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