Fast and Sensitive Detection of SARS-CoV-2 Nucleic Acid Using a Rapid Detection System Free of RNA Extraction

Table 7

Comparison with other COVID-19 nucleic acid system¹⁾.

Names	Flash20 SARS-CoV-2 detection system	Xpert Xpress SARS-CoV-2 test	Visby medical COVID-19 point of care test	DiaCarta QuantiVirus™ SARS-CoV-2 Test Kit
Test type	POCT	POCT	POCT	Conventional PCR
Method	RT-PCR	RT-PCR	RT-PCR	RT-PCR
Sample type	Nasopharyngeal and oropharyngeal	Nasopharyngeal, oropharyngeal, nasal, mid-turbinate swab, and nasal wash/aspirate specimens	Nasopharyngeal, anterior nasal, and mid-turbinate swabs	Upper respiratory specimen sputum
Time from sample to answer	30 min	45 min	30 min	>2 hours
LoD	250 copies/mL	0.0200 PFU/mL	435 copies/swab	200 copies/mL or 100 copies/mL^1)
Sensitivity	98%	97.80%	100%	100%
Specificity	100%	95.60%	95%	100%
Target gene	ORF1ab and N	N2 and E	N	ORF1ab, N, and E

¹⁾The LoD of DiaCarta QuantiVirus™ SARS-CoV-2 Test Kit is different in detecting instrument, 200 copies/mL for ABI QuantStudio 5 and ABI 7500 Fast Dx, and 100 copies/mL for Bio-Rad CFX384.