Transcription Factor E2F1 Regulates the Expression of ADRB2

Figure 2

(a) Prediction of binding sites of E2F1 using JASPAR. (b) Phylogenetic conservation analysis of the E2F1 binding sites on human ADRB2 gene promoter. (c) The mutation of the putative binding site E2F1 on the human ADRB2 promoter around 130 bp. (d–g) Regulation of ADRB2 promoter activity by E2F1 in A549 and BEAS-2B cells. The change of E2F1 level did not affect the activity of the pGL3-basic empty vector. The pGL3-basic or pGL-1879/+39 plasmids were cotransfected with pcDNA3.1 (100 ng) or pcDNA-E2F1 (100 ng) into A549 (d) and BEAS-2B (f) cells. Luciferase activity was detected 24 h following transfection (, ). At the same condition, the pGL3-basic or pGL-1879/+39 plasmids were cotransfected with si-NC (100 nM) or si-E2F1 (100 nM) into A549 (e) and BEAS-2B (g). Luciferase activity was detected 24 h following transfection (). (h–k) Equal amounts of reporter plasmids of human ADRB2 promoter containing wild or mutant E2F1 sites were cotransfected with plasmids of E2F1 overexpression or knockdown into A549 ((h) , (i) ) and BEAS-2B ((g) , (k) ) cells. The relative luciferase activities were detected.

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