Transcription Factor E2F1 Regulates the Expression of ADRB2

Figure 4

E2F1 upregulates the expression of human ADRB2 gene. (a) BEAS-2B cells were seeded in 12-well plates at a concentration of 1.5 × 105 cells per well 24 h before pcDNA-E2F1 or si-E2F1. After 24 h, cells were harvested for RNA, and reverse-transcription was performed to obtain cDNA for qRT-PCR. GAPDH was utilized as a housekeeping gene for calibration (). BEAS-2B cells were seeded in 6-well plates at a concentration of 3.0 × 10⁵ cells per well and pretreated with the same condition, as described above. Forty-eight hours after transfection, cells were harvested and subjected to Western blot analysis. Equivalent amounts of total cell proteins were fractionated by SDS-PAGE and probed with antibodies specific for ADRB2, E2F1, and GAPDH. The band was visualized with the ECL reagent (b) and further analyzed utilizing Image J (c, , ).

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