International Journal of Analytical Chemistry

Research Article

Discrimination and Evaluation of Wild Paris Using UHPLC-QTOF-MS and FT-IR Spectroscopy in Combination with Multivariable Analysis

Table 3

Chemical constituents of PPA, PPY, PM, PV and PPS using UHPLC-QTOF-MS.
PeakCompound nameTypeObserved m/zt(Rt)Mass fragment
1Parisyunnanoside G isomerIsospirosteranol1223.585 [M-H]−4.7751077.5146 [M-H-Rha]−, 931.4593 [M-H-2Rha]−, 769.4178 [M-H-2Rha-Gal]−, 641.3581 [M-H-2Rha-Gal-C5H4O4]−, 479.3058 [M-H-2Rha-Gal-Glc-C5H4O4]−
2Paritriside APentacyclic triterpenoids765.1597 [M-H]−5.277737.1350 [M-H-CO]−, 633.1602 [M-H-CO-Ara]−, 455.1043 [M-H-COO-Ara-Glc]−
3Pseudoproto-PbFurostanol1079.4931 [M-H]−5.345948.4591 [M-H-Xyl]−, 623.1659 [M-H-Xyl-2Glc]−
4β-ecdysoneCholestanol479.3019 [M-H]−5.696461.2961 [M-H2O]−, 319.1921 [M-C7H14O4]−, 301.1824[M-C7H14O4-H2O]−, 283.1725 [M-C7H14O4-2H2O]−
524-O-Gal-(23S,24S)-spirosta-5,25 (27)-diene-1β,3β,23,24-tetrol-1-O-Xyl (1-6)-Glc (1-3)[Rha (1-2)]-Glc isomerIsospirosteranol1223.5473 [M-H]−6.0241091.5052 [M-H-Xyl]−, 945.4456 [M-H-Xyl-Rha]−
624-O-Gal-(23S,24S)-spirosta-5,25 (27)-diene-1β,3β,23,24-tetrol-1-O-Xyl (1-6)-Glc (1-3)[Rha (1-2)]-GlcIsospirosteranol1223.548 [M-H]−6.3971091.5152 [M-H-Xyl]−, 945.4583 [M-H-Xyl-Rha]−
7Parisyunnanoside GIsospirosteranol1269.5503 [M + COOH]−6.4181091.5026 [M-H-Xyl]−, 945.4307 [M-H-Xyl-Rha]−, 799.3541 [M-H-Xyl-Rha-Fuc]−, 637.3417 [M-H-Xyl-Rha-Fuc-Glc]−
8Kaempferol-3-O-Glc (1-4)-GlcFlavonoids610.2859 [M-H]−6.799448.2910 [M-H-Glc]−
97β-ol-sitosterol-3-O-GlcPhytosterol592.2584 [M-H]−7.117564.2737, 548.2576, 515.1946, 119.0345,
10Padelaoside BIsospirosteranol1355.59 [M-H]−6.8571224.6083 [M-H-Xyl]−, 1078.5370 [M-H-Xyl-Rha]−, 897.4143 [M-H-Xyl-Rha-OGlc]−
11Parisyunnanoside G isomerIsospirosteranol1223.545 [M-H]−7.0571091.4920 [M-H-Xyl]−, 945.5193 [M-H-Xyl-Rha]−
12Parisyunnanoside JC21 steroids977.4307 [M-H]−7.619845.3843 [M-H-Rha]−, 797.3667 [M-H-Rha-C2H8O]−, 665.3212 [M-H-2Xyl]−, 519.2603 [M-H-2Xyl-Rha]−
13Parisyunnanoside HIsospirosteranol1061.4873 [M-H]−7.759929.4440 [M-H-Xyl]−, 783.3871 [M-H-Xyl-Rha]−, 637.3271 [M-H-Xyl-Rha-Fuc]−
14Unkonwn 1Others479.5069 [M-H]−8.138461.2921 [M-H2O]−, 319.1955 [M-C7H14O4]−, 301.1837 [M-C7H14O4-H2O]−, 283.1706 [M-C7H14O4-2H2O]−
15Smilaxchinoside BFurostanol1195.535 [M-H]−8.641049.4931 [M-H-Rha]−, 903.4696 [M-H-2Rha]−, 741.4197 [M-H-2Rha-Glc]−, 579.2568 [M-H-2Rha-2Glc]−
16Parisyunnanoside H isomerIsospirosteranol1061.4898 [M-H]−8.816929.4470 [M-H-Xyl]−, 765.3738 [M-H-Xyl-Gal]−, 619.3119 [M-H-Xyl-Rha-Gal]−, 601.3033 [M-H-Xyl-Gal-Rha-OH]−, 439.2532 [M-H-Xyl-Rha-Gal-Glc-OH]−
17Parisyunnanoside H isomerIsospirosteranol1107.4977 [M-H]−8.846929.4452 [M-H-Xyl]−, 765.3761 [M-H-Xyl-Gal]−, 619.3161 [M-H-Xyl-Rha-Gal]−, 439.2543[M-H-Xyl-Rha-Gal-Glc-OH]−
18Polyphyllin H isomerFurostanol1109.5073 [M + COOH]−9.17931.4595 [M-H-Xyl]−, 785.4023 [M-H-Xyl-Rha]−,623.3481 [M-H-Xyl-Rha-Glc]−, 477.2890 [M-H-Xyl-2Rha-Glc]−
19Parisyunnanoside G isomerIsospirosteranol1223.5794 [M-H]−9.2241139.5201 [M-H-C5H14]−, 977.4672 [M-H-C5H14-Glc]−
20Polyphylloside IVIsospirosteranol1061.5255 [M-H]−9.451916.4755 [M-H-Rha]−, 770.4132 [M-H-Rha]−
21Chonglouoside SL-18Isospirosteranol931.4614 [M-H]−9.636903.4320 [M-H-CO]−, 757.3472 [M-H-CO-Rha]−
22Polyphyllin GFurostanol1049.524 [M-H]−11.445903.4645 [M-H-Rha]−, 757.3544 [M-H-2Rha]−, 595.3110 [M-H-2Rha-Glc]−, 433.1617 [M-H-2Rha-2Glc]−
23Polyphylloside IV isomerIsospirosteranol1061.5263 [M-H]−14.781929.4769 [M-H-Gal]−, 765.4073 [M-H-Gal-Rha]−, 619.0835 [M-H-Gal-2Rha]−, 439.1617 [M-H-Gal-2Rha-OGlc]−
24Polyphylloside III/parisaponin IIsospirosteranol1093.5511 [M + COOH]−14.953901.4830 [M-H-Rha]−, 755.4256 [M-H-2Rha]−, 593.3341 [M-H-2Rha-Glc]−
25Pennogenin-3-O-Glc-(1-5)-Ara (1-4) [Rha (1-2)]-GlcIsospirosteranol1033.5297 [M-H]−15.231901.4856 [M-H-Ara]−, 755.4224 [M-H-Ara-Rha]−, 593.3732 [M-H-Ara-Rha-Glc]−, 431.1308 [M-H-Ara-Rha-2Glc]−
26Polyphyllin HFurostanol1063.5417 [M-H]−15.551917.4765 [M-H-Rha]−, 901.4845 [M-H-O-Rha]−, 755.4325 [M-H-O-2Rha]−, 593.3737 [M-H-O-2Rha-Glc]−, 431.3246 [M-H-O-2Rha-2Glc]−
27Parispolyside ECholestanol1073.5248 [M + COOH]−16.493893.4583 [M-H-Ara]−, 747.3036 [M-H-Ara-Rha]−, 585.3418 [M-H-Ara-Rha-Glc]−
28Chonglouoside SL-3Isospirosteranol1063.539 [M-H]−16.524901.4854 [M-H-Glc]−, 755.4158 [M-Glc-Rha]−, 609.3327 [M-Glc-2Rha]−, 447.2354 [M-2Glc-2Rha]−
29Chonglouoside SL-4Isospirosteranol1063.5428 [M-H]−17.274901.4424 [M-H-Glc]−, 755.3874 [M-Glc-Rha]−, 609.3722 [M-Glc-2Rha]−
30Parispolyside E isomerCholestanol1027.419 [M-H]−17.72929.4458 [M-H-C5H12O]−, 783.3883 [M-H-C5H12O-Rha]−, 637.3274 [M-H-C5H12O-2Rha]−,473.2534 [M-H-C5H12O-2Rha-Glc]−
313β-ol-oleane-12-en-28-oic acid- 3-O-Glc (1-2)-GlcPentacyclic triterpenoids845.3762 [M + COOH]−17.95637.3279 [M-H-Glc]−, 475.3160 [M-H-2Glc]−
32Parispseudoside BCholestanol893.2173 [M-H]−19.085747.2188 [M-H-Rha]−, 585.1578 [M-H-Rha-Glc]−
33Hypoglaucin HC21 steroids767.2189 [M-H]−19.101621.4030 [M-H-Rha]−
34Glyceryl α-mono-palmitateOthers329.2345 [M-H]−19.303247.2143, 229.1468, 211.1371, 171.1031, 139.1144
35Parisaponin IIsospirosteranol1047.5331 [M-H]−20.677885.4764 [M-H-Glc]−, 739.4232 [M-H-Glc-Rha]−, 593.3678 [M-H-Glc-2Rha]−, 431.1245 [M-H-2Glc-2Rha]−
36Parispseudoside ACholestanol1229.6029 [M + COOH]−20.8761037.5500 [M-H-Rha]−, 891.4866 [M-H-2Rha]−, 745.4190 [M-H-3Rha]−
37(8R,9R,10S,6Z)-triol-octadec- 6-enoic acid/glyceryl α-mono-palmitateOthers329.2348 [M-H]−22.429 
387β-ol-sitosterol-3-O-GlcPhytosterol541.3452 [M-H]−23.046379.2784 [M-H-Glc]−
39Polyphyllin VIIIsospirosteranol1029.5424 [M-H]−25.37883.4873 [M-H-Rha]−, 737.4273 [M-H-2Rha]−, 591.9858 [M-H-3Rha]−, 429.1363 [M-H-3Rha-Glc]−
40Cussonoside B/pregna-5,16-diene-3B-ol-20-one-3-O-Rha (1-2)-Rha (1-2)-[Rha (1-4)]-GlcPentacyclic triterpenoids915.4648 [M-H]−25.898737.4158 [M-H-O-Glc]−, 591.3557 [M-H-O-Glc-Rha]−
41MyrincitrinFlavonoids313.241 [M-H]−28.267277.2196 [M-H-CH2O]−
42Polyphyllin IIIsospirosteranol1013.5392 [M-H]-29.057867.4844 [M-H-Rha]−, 721.4232 [M-H-2Rha]−, 575.3578 [M-H-3Rha]−, 413.2257 [M-H-3Rha-Glc]−
43Polyphyllin IIIIsospirosteranol883.4812 [M-H]−29.411737.4196 [M-H-Rha]−, 721.4225 [M-H-O-Rha]−, 575.3619 [M-H-2Rha]−, 413.1488 [M-H-2Rha-Glc]−
44Polyphyllin IIsospirosteranol853.4645 [M-H]−29.541721.4166 [M-H-Ara]−, 575.3624 [M-H-Ara-Rha]−, 413.0833 [M-H-Ara-Rha-Glc]−
45Polyphyllin VOthers723.3857 [M-H]−30.011575.3649 [M-H-Rha]−, 397.1376 [M-H-Rha-Glc]−
467-O-Rha-kaempferol-3-O-GlcFlavonoids595.2918 [M-H]−30.165415.2354 [M-H-Glc]−
474,2′,4′-trihydroxy-chalconeFlavonoids255.2347 [M-H]−35.700237.2208 [M-H-OH]−