Simultaneous Determination and Stability Analysis of Ten New Psychoactive Substances including Synthetic Cathinones, Phenethylamines, and Ketamine Substitutes in Urine Using Liquid Chromatography-Tandem Mass Spectrometry

Table 1

Time program for the chromatographic separation procedure.

Total time (min)	Flow rate (mL/min)	Mobile phase solvent A (%)	Mobile phase solvent B (%)
0	0.4	95	5
1	0.4	95	5
4	0.4	70	30
7	0.4	50	50
13.5	0.5	5	95
14	0.5	5	95
14.5	0.4	95	5
16	0.4	95	5

Solvent A: 97.9% water with 2% methanol and 0.1% formic acid. Solvent B: 99.9% methanol with 0.1% formic acid.