Simultaneous Determination and Stability Analysis of Ten New Psychoactive Substances including Synthetic Cathinones, Phenethylamines, and Ketamine Substitutes in Urine Using Liquid Chromatography-Tandem Mass Spectrometry

Table 2

Retention time, multiple reaction monitoring transitions, dwell time, cone voltage, collision energy for analytes, and internal standards.

Compound name	Retention time (min)	Precursor ion (m/z)	Product ion (m/z)	Dwell time (ms)	Cone voltage (V)	Collision energy (V)	Internal standard
Mephedrone	5.38	178.00	160.00	0.003	18	12	Mephedrone-D3
			145.00		18	22
Butylone	5.54	222.10	174.10	0.003	25	15	Butylone-D3
			204.10		30	10
Pentylone	6.47	236.00	188.00	0.008	24	20	Butylone-D3
			218.00		24	12
Eutylone	6.01	236.00	188.00	0.008	36	25	Butylone-D3
			218.00		36	19
Ephylone	6.89	250.00	202.00	0.008	31	26	Butylone-D3
			174.00		31	41
4-MEAPP	7.43	220.00	105.00	0.022	12	26	Butylone-D3
			144.00		12	38
PMA	4.60	166.00	149.00	0.003	25	8	MDA-D5
			121.00		25	20
PMMA	5.15	180.10	120.91	0.003	30	22	MDA-D5
			91.10		30	30
DCK	6.04	204.00	173.00	0.008	21	17	Ketamine-D4
			145.00		21	24
2-FDCK	5.88	222.00	109.00	0.008	22	25	Ketamine-D4
			163.00		22	25
Mephedrone-D3	5.37	180.79	147.89	0.003	40	18	—
			144.83		38	20
Butylone-D3	5.53	225.03	177.07	0.003	36	18	—
			148.94		36	26
MDA-D5	4.47	185.00	168.00	0.003	20	10	—
			110.00		20	20
Ketamine-D4	6.59	242.00	129.00	0.008	20	30	—
			224.00		20	20

LC-MS/MS: liquid chromatography-tandem mass spectrometry; mephedrone: 4-methylmethcathinone; ephylone: N-ethylpentylone; 4-MEAPP: 4-methyl-α-ethylaminopentiophenone; PMA: para-methoxyamphetamine; PMMA: para-methoxymethamphetamine; DCK: deschloroketamine; 2-FDCK: 2-fluorodeschloroketamine; MDA-D5: 3,4-methylenedioxyamphetamine-D5.