Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 7
Correlation between Aβ-FAM binding and the levels of cholesterol in the membrane over a wide range of membrane cholesterol. To vary the levels of cholesterol in the cell membrane, PC12 cells were treated with different concentrations of methyl-β-cyclodextrin (CD) (a), mevastatin (), (b) and polyoxyethanyl-cholesteryl sebacate (Cholesterol) (c). Then, cells were exposed for 80 minutes to Aβ-FAM and the fluorescence from the membrane bound Aβ-FAM was evaluated by flow cytometry. The percentage of cells in gate (numbers in the figures) shows that enriching or depleting the cell membrane of cholesterol increases or reduces the levels of Aβ-FAM binding to the surface of the membrane. The plot in (d) compiles the percentage of Aβ + cells as a function of the level of cholesterol in the membrane recorded at the time when membrane-bound Aβ-FAM was measured. The straight line is the result of a linear relation fit of data ().
