Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer

Table 2

Effect of extracellular matrix on differentiation of xenografts.

MEC ^1	SC^2	ECM	𝑁^3	𝑥/𝑇^4	Outcome
None	hTert MSC (2 × 10^5)	Matrigel orCol I plus matrigel (3 : 1)	8	—	No growth or no xenogratfs
MCF7 (2 × 10^5)	None	Col I plus matrigel (3 : 1)	8	0/16	Solid tumor
MCF7/CEACAM1-4S (2 × 10^5)	None	Col I plus matrigel (3 : 1)	8	0/16	Solid tumor and no differentiation
MCF7 (2 × 10^5)	hTert MSC (2 × 10^5)	None	8	0/4	Solid tumor and low engraftment
MCF7/CEACAM1-4S (2 × 10^5)	hTert MSC (2 × 10^5)	None	8	0/3	Solid tumor and low engraftment
MCF7 (2 × 10^5)	None	Matrigel	8	0/16	Solid tumor
MCF7/CEACAM1-4S (2 × 10^5)	None	Matrigel	8	15/16*	Differentiation into acini

¹Mammary epithelial cellline (number of cells injected), ²Stromal cell line (number of cells injected), ³Number of animals, ⁴Number of xenografts exhibiting gland-like structures (𝑥)/Total number of xenografts (𝑇), *indicates statistical significance (𝑃<.01) for differentiation of xenografts, when compared with all other groups in this table.