Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer

Table 4

Effect of type and number of stromal cells on differentiation of xenografts.

MEC ^1	SC^2	ECM	𝑁^3	𝑥/𝑇^4	Outcome
MCF7 (2 × 10^5)	hTert MSC (2 × 10^5)	Matrigel	8	0/16	Solid tumor
MCF7/CEACAM1-4S (2 × 10^5)	hTert MSC (2 × 10^5)	Matrigel	8	15/16*	Differentiation into gland like structures
MCF7/CEACAM1-4S (2 × 10^5)	hTert MSC (5 × 10^4)	Matrigel	8	16/16*	Differentiation into gland like structures
MCF7/CEACAM1-4S (2 × 10^5)	RMF/EG (2 × 10^5)	Matrigel	8	0/16	Fibrosarcoma
MCF7 (2 × 10^5)	RMF/EG (2 × 10^5)	Matrigel	8	0/16	Fibrosarcoma

¹Mammary epithelial cell line (number of cells injected), ²Stromal cell line (number of cells injected); ³Number of animals, ⁴Number of xenografts exhibiting gland-(acinar-) like structures (𝑥)/Total number of xenografts (𝑇), *indicates statistical significance (𝑃<.01) for differentiation of xenografts, when compared with control group (MCF7).