Expression of the Classical and Nonclassical HLA Molecules in Breast Cancer
Figure 1
Immunoperoxidase labeling of HLA-Ia, HLA-II, HLA-G, and HLA-E in cytoplasmatic membranes of IDC breast tumor cells and controls. (a) Positive control for HLA-I, HLA-DR, and HLA-DQ expression in human tonsil slides, showing immunolabeling in >10%. (b) Negative control, human tonsil slides without primary antibodies, showing absence of immunolabeling. (c) HLA-I expression in IDC classified as positive, showing brown immunolabeling in >10%. (d) HLA-DQ expression in IDC classified as positive, showing intense brown immunolabeling in >10%. (e) HLA-DR expression in IDC classified as positive, showing intense brown immunolabeling in >10%. (f) Positive control for HLA-G and HLA-E expression in human trophoblast slide, showing immunolabeling in >25%. (g) Negative control, human trophoblast slide without primary antibodies, showing absence of immunolabeling. (h) HLA-G expression in IDC classified as positive, presenting labeling in >25%. (i) HLA-E expression in IDC classified as positive, showing labeling in >25%. Bar scale 250 μm.
