Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines

Figure 5

Western blot analyses for tropomyosin protein expressed in various breast epithelial cell lines. Top panel is the western blot stained with Ponceau dye. Middle panel is the blot stained with anti-tropomyosin antibody TM311 that reacts with all tropomyosins containing exon 1a. Bottom panel is the western blot stained with anti-tropomyosin antibody CH1 that reacts with all tropomyosins containing exon 9a. Lane M, molecular weight marker; lane 1, MDAMB453; lane 2, HCC1419; lane 3, HCC1806; lane 4, MDAMB157; lane 5, BT474; lane 6, HCC1187; lane 7, HCC1143; lane 8, MDAMB468; lane 9, MCF7; lane 10, MCF10A; lane 11, HCC1143BL; lane 12 HCC1187BL; lane 13, skeletal muscle.