In Situ Real-Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytes

Figure 2

In situ real-time CL imaging of basal and BPQ-stimulated ROS formation in cultured monolayers of H9c2 cardiomyocytes. H9c2 cells were cultured in 6-well plates. Immediately prior to CL imaging, confluent cells in culture were washed once with PBS followed by addition of 2 mL PBS containing 1 𝜇M BPQ or other reagents, as described under Materials and Methods section. (a) Representative CL images acquired at the indicated time points; the images for the first 10 minutes were acquired with a 5-minute delay; (b) layout of treatment groups; probe refers to luminol/HRP; (c) quantification of time-dependent ROS formation by luminol/HRP-amplified CL imaging. Data in (c) represent averages of two measurements.