In Situ Real-Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytes

Figure 4

In situ real-time CL imaging of ROS formation in cultured H9c2 cardiomyocytes exposed to inhibitors of mitochondrial ETC. H9c2 cells were cultured in 6-well plates. Immediately prior to CL imaging, confluent cells in culture were washed once with PBS followed by addition of 2 mL PBS containing 10 𝜇M AA or ROT in the presence of probe (luminol/HRP), as described under Materials and Methods section. (a) Representative CL images acquired at the indicated time points; (b) layout of treatment groups, probe refers to luminol/HRP, (c) quantification of time-dependent ROS formation by luminol/HRP-amplified CL imaging. (c) Shows the quantitative data of the images in (a).