Research Article
Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix
Figure 5
Three-dimensional images of collagen I stained using immunofluorescence in fibrotic lungs after ClearT2 tissue-clearing treatment. (a) Three-dimensional images reconstructed from single-plane images. Each 5 μm stack is 20–120 μm below the lung surface. (b) The image of cross-sections indicated by the red lines in (a). (c) Single-plane images at the indicated depth (μm) from the lung surface. Green fluorescence indicates the localization of collagen I. The scale bar is 100 μm.
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