X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT

Figure 2

Evaluating the potential of the different CESA solutions for cryo-CECT (isopentane −78°C). Transverse cryo-CECT slices of kidneys that were stained by either one of the CESA solutions (Hf-WD POM, Lugol PBS, or B-Lugol) and, subsequently, frozen by submersion in isopentane at −78°C. A magnification (white rectangle) of the cortex is shown below. Hf-WD POM and B-Lugol allowed the visualization of glomeruli (red arrow) and tubuli (blue arrow). Image histograms were windowed based on their dynamic range. Hence, images are not displayed as normalized grey values in-between different datasets.