X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT

Table 1

MicroCT acquisition parameters.

Acquisition parameters	Overview	Central zoom	Cortical zoom	Upper pole (TRAKI)
MicroCT system	GE Nanotom M	GE Nanotom M	UniTOM HR	GE Nanotom M
Voxel size (m)	8	3	1.5	2
Tube voltage (kV)	60/80	80	80	90
Tube power (W)	—	—	3	—
Tube current (A)	250	155	—	90
Nr. of projections	1200	2400	2800	2100
Exposure time (ms)	500	500	500	750
Average	1	2	4	2
Skip	—	1	—	1
Acquisition time (min)	10	60	105	80
Source-detector-distance (mm)	500	333	495	450
Source-sample-distance (mm)	40	10	15	9
CECT or cryo-CECT	Both	Both	Cryo-CECT	Cryo-CECT
Grey value normalization	Yes	No	No	No

Corresponds to tube voltage (60 kV) used for the image acquisition of unstained kidney.