In Vitro Osteogenic Properties of Two Dental Implant Surfaces

Table 1

Sense (S) and antisense (A) sequences of the primers used in the real-time PCR of target and housekeeping genes.

Gene	Primer sequence (5′ to 3′)		GeneBank; accession number	Amplicon size (base pairs)
ALP	S	AACCCAGACACAAGCATTCC	X13409	151
	A	GAGAGCGAAGGGTCAGTCAG
BSP	S	GAAAATGGAGACGGCGATAG	L20232	141
	A	ACCCGAGAGTGTGGAAAGTG
CD44	S	CTTCCATCTTGACCCGTTGT	XM_283773	175
	A	ACAGTGCTCCTGTCCCTGAT
Coll-I	S	AGAGCATGACCGATGGATTC	NM_007742	177
	A	CCTTCTTGAGGTTGCCAGTC
Dlx2	S	AGTTCGTCTCCGGTCAACAA	NM_010054.1	125
	A	GCCGCCAGCTGGAAACTGGA
Hes1	S	CTGCAGCGGGCGCAGATGAC	NM_008235.2	114
	A	ACACGTGGACAGGAAGCGGG
IGF-I	S	GCTCTTCAGTTCGTGTGTGG	U75390	142
	A	ACATCTCCAGCCTCCTCAGA
IL-6	S	CCGGGAGCAGTGTGAGCTTA	NM_031168	171
	A	TAGATGCGTTTGTAGGCGGTC
OPG	S	AGACCATGAGGTTCCTGCAC	U94331	131
	A	AAACAGCCCAGTGACCATTC
Osx	S	ACTGGCTAGGTGGTGGTCAG	NM_007419	135
	A	GGTAGGGAGCTGGGTTAAGG
RANKL	S	GGCCACAGCGCTTCTCAG	AF019048	141
	A	TGACTTTATGGGAACCCGAT
Smad1	S	ATGCCAGCTGACACACCCCC	NM_008539.3	112
	A	TTTCAGCGGGCAGTGGAGGC
Smad5	S	GGAGTTTGCTCAGCTTCTGG	NM_008541.2	134
	A	TGGTGACGTCCTGTCGGTGGT
18S rRNA	S	GTAACCCGTTGAACCCCATT	X00686	151
	A	CCATCCAATCGGTAGTAGCG
GAPDH	S	ACCCAGAAGACTGTGGATGG	XM_132897	171
	A	CACATTGGGGGTAGGAACAC