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| Denture cleansers/manufacturer | Generic description | Composition | Preparation and immersion instructions | Immersion protocol |
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| Distilled water (W) | Distilled water | | (i) Immersion for the whole duration of the experiment at room temperature | (i) Baseline measurement was done using specimens with different levels of nano-ZrO2 reinforcement immersed in distilled water for 2 days (T0)
(ii) Then, all specimens were immersed in respective solutions for 180 days followed by measurements (T1).
(iii) The immersion continued for further 185 days to simulate 1 year of clinical use and final measurements were made (T2).
(iv) After immersion in solutions, resin specimens were retrieved, thoroughly washed under running water, and stored in distilled water at room temperature until the next immersion cycle the following day.
(v) The immersion procedure was performed by one investigator where fresh solutions were prepared daily for 365 days. |
| Corega (C)/GlaxoSmithKline; Stafford Miller, Ireland; Lot no. XL9X | Disinfectant effervescent tablet | Potassium monopersulfate; sodium bicarbonate; sodium lauryl sulfoacetate; sodium perborate monohydrate; sodium polyphosphate | (i) One tablet dissolved in 200 mL of warm tap water (40°C)
(ii) Immersion for 3 minutes |
| Sodium hypochlorite-NaOCl (S) | Sodium hypochlorite solution | Sodium hypochlorite solution, 1% active chlorine | (i) Solution of 5.25% sodium hypochlorite (NaOCL, 1:5 dilution) was diluted to obtain 1% sodium hypochlorite by adding 50 mL NaOCl to 200 mL water.
(ii) Immersion for 10 minutes at room temperature |
| Renew (R)/Mid Continental dental Supply Co. LTD., Winnipeg, Canada; Lot no. 144 | Disinfectant effervescent powder | Sodium hypochlorite, sodium bicarbonate; salt and lemon; sodium perborate; sodium phosphate; monosodium persulfate; ethylene diamine tetraacetic acid (EDTA) | (i) One teaspoon dissolved in 200 mL of warm tap water (40°C)
(ii) Immersion for 20 minutes |
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