Development of an Electroactive Hydrogel as a Scaffold for Excitable Tissues

Figure 5

Stained images of cell-seeded hydrogels after actuation. (a) MSCs stimulated at 20 V, (b) 10 V, and (c) 5 V. Cells were stimulated after reaching confluency, stained at 24 hours, and observed at 10x magnification. Stimulation above 5 V appeared to dislodge all cells from hydrogels (A and B). All images were taken with 2 fluorescent channels that labeled cells live (green, ex/em; ∼450/475 nm) and dead (red, ex/em; ∼600/635 nm).

(a)

(b)

(c)