Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats

Table 1

Oligonucleotide primers.

Genes	Primer sequence (5′-3′)	Sequence amplified	Product size (bp)
KL	F_GAATACGCAAAGTAGCCACAAAG R_GAGCAAGACTCACTGAGGATG	XM_039089809.1	104
FSHR	F_ATCATTTGCTGGATTTGGAGAC R_TTGGGTAGGTTGGAGAACACAT	NM_199237.2	96
GDF-9	F_CAATACCGTCCGGCTCTTCAG R_AGGGGTCCTGTCATCTGGTTG	XM_017597502.2	72
FIGLA	F_CAGGAAGCCCAGTAAAGTTGA R_CTTCTTTCTTCAAGCCACTTGC	XM_575589.7	218
BCL-2	F_CGGGAGAACAGGGTATGA R_CAGGCTGGAAGGAGAAGA	NM_016993.2	149
BAX	F_CAGGACGCATCCACCAAGAAG R_TGCCACACGGAAGAAGACCTC	NM_017059.2	135
B-ACTIN	F_ CCC​ATC​TAT​GAG​GGT​TAC​GC R_TTTAATGTCACGCACGATTTC	NM_031144.3	150

F: forward, R: reverse, KL: klotho, FSHR: follicle-stimulating hormone receptor, GDF-9: growth differentiation factor-9, FIGLA: factor in germ line alpha, BCL-2: B-cell lymphoma 2, BAX: BCL-2-like protein 4, and B-ACTIN: beta-actin.