Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes
Figure 1
The density of nucleosomes at the p21 promoter nucleosome is not altered in the absence of SRCAP or p400. A549 cells were transfected with control, SRCAP or p400 siRNA and harvested 72 hours later. In (a), DNA was isolated from mononucleosomes and amplified by qPCR using overlapping primers tiling the p21 promoter (see Table S1(c)) and presented relative to the amount of DNA amplified at position −2249. The graph represents the mean result and standard error of three or more independent ChIP experiments. In (b), knockdown of SRCAP or p400 protein, compared to control-transfected cells, was confirmed by Western blot. Beta actin was used as a loading control.
