Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes
Figure 2
Knockdown of SRCAP or p400 decreases H2A.Z deposition into p21 promoter nucleosomes. A549 cells were transfected with control, SRCAP or p400 siRNA and harvested 72 hours later. In (a), nucleosome ChIP assays were performed using anti-H2A.Z antibody and immunoprecipitated DNA was amplified by qPCR using the indicated primer sets (see Table S1(c)). The amount of DNA amplified at each position is presented relative to the amount of DNA amplified at position −2249. The graph represents the mean result and standard error of three or more independent ChIP experiments. In (b), histones were acid-extracted and protein levels were determined by Western blot analysis using anti-H2A.Z antibody. Histone H3 was used as a loading control.
