Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes

Figure 4

The density of nucleosomes at the Sp1 promoter is not altered in the absence of SRCAP or p400. A549 cells were transfected with control, SRCAP or p400 siRNA and harvested 72 hours later. DNA was isolated from mononucleosomes and amplified by qPCR using overlapping primer sets tiling the Sp1 promoter (see Table  S2) and presented relative to the amount of DNA amplified at position −1241. The graph represents the mean result and standard error of three or more independent ChIP experiments.