Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes

Figure 5

SRCAP and p400 regulate H2A.Z deposition equivalently at Sp1 promoter nucleosomes. 549 cells were transfected with control, SRCAP, or p400 siRNA and harvested 72 hours later. Mononucleosome ChIP assays were performed using anti-H2A.Z antibody and immunoprecipitated DNA was amplified by qPCR using the indicated primer sets (see Table  S2). The amount of DNA amplified at each position is presented relative to the amount of DNA amplified at position −1241. The graph represents the mean result and standard error of three or more independent ChIP experiments.