Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes

Figure 6

SRCAP mediates transcriptional activity independent of H2A.Z deposition. HeLa cells were transiently transfected with 300 μg of Sp-1-luciferase reporter gene plasmid or the control pGL2 luciferase reporter gene plasmid and, where indicated, with 1000 ηg of plasmid expressing wild-type SRCAP or the SRCAP_∆ATP mutant or the control vector pcDNA 3.1. The relative luciferase activity is reported compared to the luciferase activity observed in cells transfected with pGL2- luciferase and pcDNA 3.1. The graph represents the mean result and standard error of three or more independent transfection experiments.