Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1
Figure 12
Roscovitine reverses the effects of CKIγ2 overexpression on and actin stress fibers. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against total and phospho-Ser10 , Cdk5, and HA. β-Actin was used as loading control. (b) Equivalent amount of total cell lysate proteins from indicated cells treated or not with roscovitine that were subjected to Western Blot analysis using specific antibodies against or β-actin was used as loading control. (c) Cells overexpressing CKIγ2 were incubated with roscovitine (25 μM, 16 hrs) before to be stained with phalloidin to visualized actin organization. Images were taken at 63X.
