|
| Serial No. | Genes | Annealing temperature (°C) | Product size (bp) | Primer sequences |
|
| | Chick | | | |
| 1 | Asporin | 55.4 | 328 | F: 5-GCAACATTCCACCAGATAC-3
R: 5-AGAGGATTTGCACTCATTTC-3 |
| 2 | Myh7 | 53.8 | 156 | F: 5-CCCCTCAATGAGACAGTGGT-3
R: 5-GGCTGAGACAGTCTGGAAGG-3 |
| 3 | Gata4 | 59.2 | 360 | F: 5-GTGTCACCTCGCTTCTCCTT-3
R: 5-GTGCCCTGTGCCATCTCT-3 |
| 4 | Col1a1 | 52.6 | 173 | F: 5-GTACCTCAGCAAGAACCCCA-3
R: 5-AGTGGTAGGTGACGTTCTGG-3 |
| 5 | Sm22α | 52.6 | 198 | F: 5-ATGTTCCAGACCGTTGACCT-3
R: 5-GCCAATGATGTTCTTGCCCT-3 |
| 6 | Vimentin | 53.8 | 201 | F: 5-CCAGATGCGTGAAATGGAGG-3
R: 5-CTCTCTTCTCCCTCCAGCAG-3 |
| 7 | Fibronectin | 56.9 | 232 | F: 5-AGATGTTCCAAGGGACCTGG-3
R: 5-TCCACGACCAGTTACAGCAT-3 |
| 8 | Alp | 53.8 | 248 | F: 5-GCTAAGGATGAAGGCAAGGC-3
R: 5-TACTCCACATCGCTGGTGTT-3 |
| 9 | N-Cadherin | 57.6 | 219 | F: 5-TTGGCTAAGGGGATTCAGCA-3
R: 5-TGCAGGGTCCGAAAGTTTTG-3 |
| 10 | Adamts5 | 50 | 183 | F: 5-TCCTAGAAACAATGGCCGGT-3
R: 5-ACTCCAGCATACTTGGGGAC-3 |
| 11 | Adamts9 | 52 | 152 | F: 5-ATTTGGATCCTGGACACCGT-3
R: 5-GTGTCACGGTGCACATTTCT-3 |
| 12 | Osteopontin | 59.2 | 198 | F: 5-GCAGCAGACACAGAATGACC-3
R: 5-TCTGTGGGGAAGTCTGTGAC-3 |
| 13 | Runx2 | 61.1 | 211 | F: 5-CCCCGGCTCCTCCCAAAACCAA-3
R: 5-CCGCCTCCACACCGTCACTCTG-3 |
| 14 | Msx2 | 53.8 | 162 | F: 5-TCCCTTTCCCCATCAACTCC-3
R: 5-TCACCGTGCCTTTCTGATCT-3 |
| 15 | Osterix | 50.5 | 203 | F: 5-CTATAGGGGCGGTTCGGG-3
R: 5-CTCGGGTCGGTGTTATGGAT-3 |
| 16 | β-Catenin | 56.1 | 205 | F: 5-ACTGTTCTACGCCATCACCA-3
R: 5-ACCACTGGCCAGAATGATGA-3 |
| 17 | Wnt3a | 58 | 211 | F: 5-GTGAGGACGTGGAGTTT-3
R: 5-GAAGCGCTGTCGTATTTG-3 |
| 18 | Dkk1 | 51.9 | 166 | F: 5-CCGGAGCAGAAGGTTGTTTC-3
R: 5-CTGGAAACTCAGCGCGTAC-3 |
| 19 | Bmp2 | 55 | 238 | F: 5-GTTTGTGGTGGAGGTGGTTC-3
R: 5-GTCCACATACAACGGATGCC-3 |
| 20 | Smad1 | 51 | 323 | F: 5-GCAGGGAGATGAAGAAGA-3
R: 5-GCTTGTAGTGGTAAGGATTG-3 |
| 21 | Smad5 | 51 | 218 | F: 5-GGAAACAAGGAGATGAAGAG-3
R: 5-CCAGACCCGACAGTAAA-3 |
| 22 | Smad8 | 50 | 254 | F: 5-ACACCAGGAGACACATAG-3
R: 5-CATCTTCGTCAGCTCATATAC-3 |
| 23 | Notch1 | 53.8 | 233 | F: 5-GACATCGATGAGTGCAACCC-3
R: 5-TCCAGGTTGATCTCGCAGTT-3 |
| 24 | Sox9 | 56.1 | 191 | F: 5-CCGTTTTCTCCTCCCCTGAT-3
R: 5-CTCTTGAGGTCGGGTGTTCT-3 |
| 25 | Pit2 | 56 | 234 | F: 5-GCAGCAGATACATCAACTC-3
R: 5-AGACGTGTCCTTCTCTTC-3 |
| 26 | β-Actin | 55 | 139 | F: 5-AGTACCCCATTGAACACGGT-3
R: 5-ATACATGGCTGGGGTGTTGA-3 |
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