Comparative Transcriptomic Analyses of a Vero Cell Line in Suspension versus Adherent Culture Conditions
Table 4
Proposed strategies for Vero cell adaptation to suspension improvement.
Phenotype observed
Proposed improvement
Long doubling time
(i) Gene editing: targeted knock-out of FYN gene (ii) Gene editing: enhance upregulation of RHOU and ESR1 (iii) Medium formulation: upregulation of glycine, threonine, and serine intake (iv) Medium formulation: reduce folate intake
Low cell density
(i) Gene editing: enhance upregulation of adherens junction-related genes (ii) Gene editing: enhance upregulation of MYC and E2F (iii) Medium formulation: upregulation aspartate intake (iv) Medium formulation: upregulate mitochondrial 1-carbon metabolism