Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity

Table 2

The absorbance of the samples with different PMSF inhibitor concentrations.

OD (280 nm)
𝑋=(𝑉𝑖/𝑉𝑜)	Time (min)	𝑆1=0.75%	𝑆2=1%	𝑆3=1.25%	𝑆4=1.5%
0.001	1	0.637	0.643	0.616	0.750
	2	0.652	0.658	0.675	0.812
	3	0.713	0.715	0.694	0.834
	4	0.739	0.760	0.767	0.924
	5	0.785	0.808	0.808	0.974
0.002	1	0.477	0.483	0.487	0.533
	2	0.514	0.525	0.533	0.540
	3	0.540	0.543	0.571	0.579
	4	0.578	0.600	0.619	0.675
	5	0.608	0.634	0.655	—
0.0075	1	0.371	0.374	0.375	0.379
	2	0.392	0.399	0.405	0.410
	3	0.407	0.416	0.424	0.430
	4	0.433	0.445	0.456	0.467
	5	—	—	0.477	—
0.025	1	0.256	0.258	0.259	0.259
	2	0.267	0.272	0.273	0.277
	3	0.272	0.276	0.280	0.284
	4	0.287	0.291	0.297	0.302
	5	—	—	—	—