Peach Kernel Extracts Inhibit Lipopolysaccharide-Induced Activation of HSC-T6 Hepatic Stellate Cells

Figure 3

Peach kernel extracts inhibit LPS-induced Smad phosphorylation and nuclear translocation. HSC-T6 cells were treated with indicated doses of peach kernel extracts for 24 h. Cells were starved for 4 h and then stimulated with LPS for 30 min. Protein level of p-Smad and total Smad was examined by western blotting. The localization of Smad was detected by immunofluorescent. Arrows indicate Smad nuclear staining. Scale bars = 10 μm.

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