The Distribution of Cytotoxic Necrotizing Factors (CNF-1, CNF-2, CNF-3) and Cytolethal Distending Toxins (CDT-1, CDT-2, CDT-3, CDT-4) in Escherichia coli Isolates Isolated from Extraintestinal Infections and the Determination of their Phylogenetic Relationship by PFGE

Table 1

Oligonucleotide primers are used in this study.

Primer	Oligonucleotide sequence (5′–3′)	Specificity	PCR product size (bp)	References
uidA F; uidA R	ATGCCAGTCCAGCGTTTTTGC; AAAGTGTGGGTCAATAATCAGGAAGTG	uidA	102	Abid and Al-Zuwainy [7]
cnf1 F; cnf1 R	GGGGGAAAGTACAGAAGAATTA; TTGCCGTCCACTCTCACCAGT	cnf1	1111	Dubois et al., [8]
cnf2 F; cnf2 R	TATCATACGGCAGGAGGAAGCACC; GTCACAATAGACAATAATTTTCCG	cnf2	1240
cnf3 F; cnf3 R	TAACGTAATTAGCAAAGA; GTCTTCATTACTTACAGT	cnf3	757
cdt1 F; cdt1 R	CAATAGTCGCCCACAGGA; ATAATCAAGAACACCACCAC	cdt1	411	Toth et al., [9]
cdt2 F; cdt2 R	GAAAGTAAATGGAATATAAATGTCCG; TTTGTGTTGCCGCCGCTGGTGAAA	cdt2	556
cdt3 F; cdt3 R	GAAAGTAAATGGAATATAAATGTCCG; TTTGTGTCGGTGCAGCAGGGAAAA	cdt3	555
cdt4 F; cdt4 R	CCTGATGGTTCAGGAGGCTGGTTC; TTGCTCCAGAATCTATACCT	cdt4	350
chuA F; chuA R	GACGAACCA ACGGTCAGGAT; TGCCGCCAGTACC AAAGACA	chuA	279	Clermont et al., [10]
yjaA F; yjaA R	TGAAGTGTCAGGAGACGCTG; ATGGAGAATGCGTTCCTCAAC	yjaA	211
TspE4C2 F; TspE4C2 R	GAGTAATGTCGGGGCATTCA; CGCGCCAACAAAGTATTACG	TspE4C2	152