SNHG12 Promotes Autophagy by Blocking the mTOR-Primary Cilia-mTOR Loop via Activating the miR-181a-5p/miR-138-5p-INPP5E Axis in Chondrocyte

Figure 1

Silencing INPP5E activates the PI3K-Akt-mTOR signaling pathway in vitro. (a) We divided the samples into the INPP5E-low expression (N = 11) group and the INPP5E-high expression (N = 12) group in the GSE43191 dataset. Using and fold change ≥2 as screening criteria, a hub of 998 DEGs were identified. (b) KEGG analysis and (c) GSEA analysis were used to analysis the pathway mechanism involved in these 998 DEGs. (d) RT-PCR assay was used to detect the expression of INPP5E. (e) WB assay was used to detect the expression of INPP5E. (f) RT-PCR assay was used to detect the expression of PI3K, Akt, and mTOR. (g) WB assay was used to detect the expression of PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR. Notes: , , and compared with the control.

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