SNHG12 Promotes Autophagy by Blocking the mTOR-Primary Cilia-mTOR Loop via Activating the miR-181a-5p/miR-138-5p-INPP5E Axis in Chondrocyte

Figure 2

Silencing INPP5E inhibits autophagy via regulating the mTOR-primary cilia-mTOR loop in vitro. (a) RT-PCR assay was used to detect the mRNA expression of IFT88, Beclin1, MAP1LC3A, and MAP1LC3B in control, NC, and si-INPP5E-2 groups. (b) WB assay was used to detect the protein expression of IFT88, Beclin1, LC3 I, and LC3 II in control, NC, and si-INPP5E-2 groups. (c) RT-PCR assay was used to detect the mRNA expression of IFT88, Beclin1, MAP1LC3A, MAP1LC3B, PI3K, Akt, and mTOR in control and CH-treated groups. (d) WB assay was used to detect the protein expression of IFT88, Beclin1, LC3 I, LC3 II, p-PI3K, PI3K, p-Akt, Akt, p-mTOR, and mTOR in control and CH-treated groups. (e) Ace-tubulin was used to label primary cilia, and LC3 II was used to label autophagy via immunofluorescence assay. Notes: , , and compared with the control or between the indicated groups.

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