SNHG12 Promotes Autophagy by Blocking the mTOR-Primary Cilia-mTOR Loop via Activating the miR-181a-5p/miR-138-5p-INPP5E Axis in Chondrocyte

Figure 3

SNHG12 promotes the expression of INPP5E via hsa-miR-181a-5p/hsa-miR-138-5p in vitro. (a) RT-PCR assay was used to detect the expression of hsa-miR-181a-5p in control, mimic-NC, and mimic-1 groups. (b) RT-PCR assay was used to detect the expression of INPP5E in control, mimic-NC, and mimic-1 groups. (c) WB assay was used to detect the expression of INPP5E in control, mimic-NC, and mimic-1 groups. (d) The dual luciferase reporting assay was used to verify the binding of INPP5E to hsa-miR-181a-5p. (e) RT-PCR assay was used to detect the expression of hsa-miR-138-5p in control, mimic-NC, and mimic-2 groups. (f) RT-PCR assay was used to detect the expression of INPP5E in control, mimic-NC, and mimic-2 groups. (g) WB assay was used to detect the expression of INPP5E in control, mimic-NC, and mimic-2 groups. (h) The dual luciferase reporting assay was used to verify the binding of INPP5E to hsa-miR-138-5p. (i) StarBase database was used to predict the lncRNAs that might bind to hsa-miR-181a-5p and hsa-miR-138-5p. (j) RT-PCR assay was used to detect the expression of SNHG12 in control, NC, si-SNHG12-1, si-SNHG12-2, and si-SNHG12-3 groups. (k) RT-PCR assay was used to detect the expression of hsa-miR-181a-5p and hsa-miR-138-5p in control, NC, and si-SNHG12-3 groups. (l, m) The dual luciferase reporting assay was used to verify the binding of SNHG12 to hsa-miR-181a-5p and hsa-miR-138-5p. Notes: , , and compared with the control.

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