SNHG12 Promotes Autophagy by Blocking the mTOR-Primary Cilia-mTOR Loop via Activating the miR-181a-5p/miR-138-5p-INPP5E Axis in Chondrocyte

Figure 5

Silencing SNHG12 activated the PI3K-Akt-mTOR signaling pathway via the hsa-miR-181a-5p/hsa-miR-138-5p-INPP5E axis in vitro. (a) RT-PCR assay was used to detect the expression of PI3K, Akt, and mTOR in control, NC, mimic-1, and mimic-1 + OE-INPP5E groups. (b) WB assay was used to detect the expression of PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR in control, NC, mimic-1, and mimic-1 + OE-INPP5E groups. (c) RT-PCR assay was used to detect the expression of PI3K, Akt, and mTOR in control, NC, mimic-2, and mimic-2 + OE-INPP5E groups. (d) WB assay was used to detect the expression of PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR in control, NC, mimic-2, and mimic-2 + OE-INPP5E groups. (e) RT-PCR assay was used to detect the expression of PI3K, Akt, and mTOR in control, NC, si-SNHG12, and si-SNHG12 + OE-INPP5E groups. (f) WB assay was used to detect the expression of PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR in control, NC, si-SNHG12, and si-SNHG12 + OE-INPP5E groups. Notes: , , and compared with the control or between the indicated groups.

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