SNHG12 Promotes Autophagy by Blocking the mTOR-Primary Cilia-mTOR Loop via Activating the miR-181a-5p/miR-138-5p-INPP5E Axis in Chondrocyte

Figure 6

Silencing SNHG12 inhibits the expression of collagen II and cyclin D1 via the hsa-miR-181a-5p/hsa-miR-138-5p-INPP5E axis in vitro. (a) RT-PCR assay was used to detect the expression of COL2A1 and CCND1 in control, NC, and si-INPP5E groups. (b) WB assay was used to detect the expression of collagen II and cyclin D1 in control, NC, and si-INPP5E groups. (c) RT-PCR assay was used to detect the expression of COL2A1 and CCND1 in control, NC, mimic-1, and mimic-1 + OE-INPP5E groups. (d) WB assay was used to detect the expression of collagen II and cyclin D1 in control, NC, mimic-1, and mimic-1 + OE-INPP5E groups. (e) RT-PCR assay was used to detect the expression of COL2A1 and CCND1 in control, NC, mimic-2, and mimic-2 + OE-INPP5E groups. (f) WB assay was used to detect the expression of collagen II and cyclin D1 in control, NC, mimic-2, and mimic-2 + OE-INPP5E groups. (g) RT-PCR assay was used to detect the expression of COL2A1 and CCND1 in control, NC, si-SNHG12, and si-SNHG12 + OE-INPP5E groups. (h) WB assay was used to detect the expression of collagen II and cyclin D1 in control, NC, si-SNHG12, and si-SNHG12 + OE-INPP5E groups. , , and compared with the control or between the indicated groups.

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