Pleural Mesothelial Cells-Induced Monocytes to the Pleural Cavity through the Effect of C3 Lytic Products in Tuberculous Pleural Effusion

Figure 4

Monocyte migration was inhibited by antibodies that blocked CR1 and CR3. (a) Representative colocalization of C3b-CR1 and C3d-CR3 in PMCs and monocytes from TPE. The cells from TPE were immunostained with C3b/C3d (red) and CR1/CR3 (green), and the nuclei were stained with DAPI (blue) (n = 4). (b) To evaluate the effect of C3b-CR1 and C3d-CR3 axes on monocytes, monocytes were seeded into the top chamber with or without anti-CR1 antibody or anti-CR3 antibody, and the supernatants from cultured PMCs were placed in the bottom chamber of the transwell system. The migratory index was calculated by dividing the number of monocytes that migrated in response to the supernatants from cultured PMCs by the number of monocytes that migrated in response to the control. vs the MO-PBS group, . ^#vs the MO-PMC group, ^## (n = 4).

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