β-Cell Specific Overexpression of GPR39 Protects against Streptozotocin-Induced Hyperglycemia
Figure 1
Overview of the generation of the tetGPR39/RIP-rtTA transgenic mice and demonstration of the β-cell specific expression of GPR39. (a) Schematic diagram of the generation of the tetGPR39/RIP-rtTA transgenic mice by crossing of the RIP-rtTA mouse with the tetGPR39 mouse. (b) QPCR specific for the transgenic FLAG-tagged human GPR39 performed on whole pancreas. (c, d) Immunohistochemistry of pancreatic islets from tetGPR39/RIP-rtTA transgenic mice, in (c, d) GPR39 was detected using the M1 anti-FLAG antibody (green). (c) Antiinsulin antibody (red). (d) Antiglucagon antibody (red).
