Influence of Secondary Hyperparathyroidism Induced by Low Dietary Calcium, Vitamin D Deficiency, and Renal Failure on Circulating Rat PTH Molecular Forms

Table 1

Evolution of biochemical parameters with various diets and partial nephrectomy.

Parameters	Normal calcium (0.5%)	Low calcium (0.1%)	Vitamin D deficiency	Moderate renal failure	Severe renal failure
N	10	12	13	7	7
Weight (g)	405±31	379±35	390±30	374±45	278±64^∗∗∗,••
Ca^++ (mmol/L)	1.30±0.02	1.28±0.03	1.24±0.02^∗∗,++	1.25±0.03	1.15±0.08^∗∗∗,•
Cat (mmol/L)	2.58±0.06	2.42±0.10^∗∗	2.42±0.09^∗∗	2.56±0.07	2.47±0.20
PO4 (mmol/L)	3.01±0.27	3.15±0.11	2.86±0.27^++	2.44±0.27^∗∗	3.10±0.57^•
Creatinine (μmol/L)	30±2	31±4	34±7	111±9^∗∗	252±84^∗∗∗,••
Alkaline phosphatase (U/L)	403±138	939±372^∗∗∗	351±177^+++	259±56	174±62^∗∗∗,•
Total protein (g/L)	52±2	54±2	55±2	51±2	45±6^•
Albumin (g/L)	23±1	23±1	24±1^+	21±1^∗∗	20±1^∗∗∗,•
25(OH)D (nmol/L)	97±18	38±3^∗	18±2^∗∗∗,+++	90±32	39±11^∗,••
1,25(OH)2D (pmol/L)	129±51	565±86^∗∗∗	58±18^∗∗∗,+++	46±10^∗∗∗	38±8^∗∗∗

Results are means ± SD. All group statistics were assessed by ANOVA, followed by Dunnett’s multiple comparison test (Kruskal-Wallis ANOVA, followed by Dunn’s comparison test for creatinine, total protein, and 25(OH)D). *𝑃<.05; **𝑃<.01; ***𝑃<.001. Unpaired T-test with or without Welch correction: Low-calcium versus vitamin D-deficient diet: ⁺𝑃<.05; ⁺⁺𝑃<.01; ⁺⁺⁺𝑃<.001. Moderate versus severe renal failure: ^•𝑃<.05; ^••𝑃<.01; ^•••𝑃<.001.