Influence of Secondary Hyperparathyroidism Induced by Low Dietary Calcium, Vitamin D Deficiency, and Renal Failure on Circulating Rat PTH Molecular Forms

Table 3

Analysis of rPTH HPLC profiles obtained by each rPTH assay.

Groups	rPTH assay	Pool value (pmol/L)	Rec. (%)	C-PTH fragments (pmol/L) (%)	Non(1-84) PTH	N-PTH	PTH(1-84)
Normal-Ca diet	Whole	3.6	117		0 (0.4)	0.3 (8.5)	3.3 (91.1)
	Total	5.4	99		0.3 (5.8)	0.4 (8.2)	4.6 (86.0)
	C	9.0	97	4.2 (46.6)	0.5 (5.2)	0.2 (2.2)	4.1 (46.0)
Low-Ca diet	Whole	7.6	111		0.2 (2.1)	0.5 (7.1)	6.9 (90.8)
	Total	11.1	94		1.7 (15.6)	0.9 (8.3)	8.4 (76.1)
	C	17.7	95	8.5 (48.3)	0.5 (2.8)	1.7 (9.6)	7.0 (39.3)
Vitamin D-deficient diet	Whole	25.5	108		0.4 (1.6)	1.5 (6.0)	23.6 (92.4)
	Total	34.0	103		3.9 (11.4)	2.7 (7.9)	27.4 (80.7)
	C	45.0	116	15.5 (34.5)	3.5 (7.7)	2.4 (5.3)	23.6 (52.5)
Moderate renal failure	Whole	22.8	114		0.4 (1.9)	7.4 (32.6)	14.9 (65.5)
	Total	15.0	108		1.3 (8.7)	3.5 (23.1)	10.2 (68.2)
	C	43.2	100	18.9 (43.8)	0.5 (1.2)	11.1 (25.8)	12.6 (29.2)
Severe renal failure	Whole	127.3	99		2.8 (2.2)	38.6 (30.3)	85.9 (67.5)
	Total	122.0	83		5.7 (4.7)	24.6 (20.2)	91.6 (75.1)
	C	278.0	107	157.9 (56.8)	0.0 (0.0)	45.0 (16.2)	75.1 (27.0)

Results of 1 HPLC profile in each case.