Liver Glucokinase_A456V Induces Potent Hypoglycemia without Dyslipidemia through a Paradoxical Induction of the Catalytic Subunit of Glucose-6-Phosphatase

Figure 5

Analysis of protein levels in livers transfected with GK and GK_A456V. Livers excised from mice in postabsorptive state were homogenized and resolved by Western blot. (a, b) Representative blots from three independent experiments. The densitometric analysis is presented (𝑁=4, *𝑃<0.05 versus pControl, ***𝑃<0.001 versus pGK_A456V). (c) Liver sections of 5-hour fasted mice injected with pControl, pGK, or pGK_A456V were immunostained with Glc6Pase antibody. TO-PRO-3 was used to visualize nuclei.

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