Liver Glucokinase_A456V Induces Potent Hypoglycemia without Dyslipidemia through a Paradoxical Induction of the Catalytic Subunit of Glucose-6-Phosphatase

Table 1

Liver metabolites of STZ-diabetic mice transfected with pControl, pGK and pGK_A456V.

	Fed			5 h-Fasted
	pControl	pGK	pGKA456V	pControl	pGK	pGKA456V
Glc (μmol/g liver)	40.70 ± 5.921	32.72 ± 1.475	47.68 ± 7.328	52.29 ± 9.524	32.23 ± 12.18	6.35 ± 4.043*
	𝑛=11	𝑛=6	𝑛=9	𝑛=5	𝑛=4	𝑛=4
G6P (μmol/g liver)	0.16 ± 0.021	0.14 ± 0.018	0.15 ± 0.021	0.06 ± 0.024	0.20 ± 0.068^§	0.04 ± 0.017
	𝑛=11	𝑛=8	𝑛=10	𝑛=5	𝑛=4	𝑛=5
F2,6BP (nmol/g liver)	1.19 ± 0.215	2.84 ± 0.625*	1.90 ± 0.517	0.71 ± 0.173	9.86 ± 1.812^§	4.14 ± 1.607
	𝑛=11	𝑛=8	𝑛=10	𝑛=5	𝑛=5	𝑛=5
Lactate (μmol/g liver)	1.31 ± 0.275	1.78 ± 0.424	2.27 ± 0.951	1.16 ± 0.239	1.28 ± 0.322	1.32 ± 0.222
	𝑛=12	𝑛=8	𝑛=10	𝑛=5	𝑛=4	𝑛=5
TAG (mg/g liver)	6.25 ± 0.26	6.08 ± 0.36	6.26 ± 0.17	9.18 ± 1.48	12.56 ± 0.50	11.84 ± 2.13
	𝑛=12	𝑛=8	𝑛=10	𝑛=5	𝑛=5	𝑛=5
GSH/GSSG ratio	11.73 ± 0.87	11.81 ± 0.93	11.37 ± 0.87	5.372 ± 0.49	9.479 ± 1.38	9.926 ± 1.84*
	𝑛=12	𝑛=8	𝑛=10	𝑛=5	𝑛=5	𝑛=5

Data are means ± SE. (𝑁=4–12). *𝑃<0.05 versus its pControl, ^§𝑃<0.05 versus fast pControl and fast pGK_A456V.