Liver GlucokinaseA456V Induces Potent Hypoglycemia without Dyslipidemia through a Paradoxical Induction of the Catalytic Subunit of Glucose-6-Phosphatase
Table 2
Gene expression profile in livers of diabetic mice after a short fast.
Gene
Protein
pControl
pGK
pGKA456V
Glycolysis and lipogenesis
Gck
GK
1.26 ± 0.72
5.28 ± 1.49
1.73 ± 0.86
Pfkfb3
PFK2
1.05 ± 0.13
1.17 ± 0.19
1.22 ± 0.19
Nr1h3
Lxr-α
1.03 ± 0.11
0.81 ± 0.03
0.91 ± 0.05
Fasn
FAS
1.14 ± 0.21
11.37 ± 5.76*
2.33 ± 0.42
Mod1
ME
1.05 ± 0.13
2.43 ± 0.91
1.57 ± 0.32
Myc
c-myc
1.07 ± 0.26
3.29 ± 1.49
0.94 ± 0.19
Srebf1
SREBP1c
1.03 ± 0.10
0.90 ± 0.16
0.85 ± 0.11
Chrebp
CHREBP
1.08 ± 0.12
2.31 ± 0.72
1.90 ± 0.29
Gluconeogenesis
Pck1
PEPCK-C
1.04 ± 0.12
0.52 ± 0.11*
0.91 ± 0.16
G6pc
Glc-6Pase
0.95 ± 0.31
1.70 ± 0.56
4.52 ± 1.39§
Hnf4
HNF-4α
1.05 ± 0.13
0.68 ± 0.07
0.77 ± 0.12
Slc2a2
GLUT-2
1.01 ± 0.05
1.20 ± 0.25
1.01 ± 0.12
Energy metabolism
Cpt1a
Cpt-1α
1.02 ± 0.09
0.83 ± 0.08*
0.92 ± 0.08
Hmgcs2
HmgCoA Synthase
1.02 ± 0.08
1.14 ± 0.22
0.91 ± 0.11
Mdh1
cMDH
1.01 ± 0.05
0.74 ± 0.04*
0.70 ± 0.10 *
Ucp2
UCP-2
1.03 ± 0.10
0.66 ± 0.02*
0.96 ± 0.15
Ppargc1
PGC-1α
1.02 ± 0.07
0.27 ± 0.07†
0.63 ± 0.14 *
Each value on the table represents the mean relative amount of mRNA with respect to that in the control experiment treatment (mean ± SE). Liver gene expression profile was analyzed by qRT-PCR using Applied Biosystems 7900HT Micro Fluidic Card and calculations. ( per group). * versus pControl; § versus pControl and pGK; † versus pControl and versus pGKA456V.
