High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function

Figure 4

PSCs-CM impairs INS-1 cell viability in vitro. PSCs-CM was supplemented into the medium, forming a concentration ratio of: 0, 25, 50 and 75%. Incubation of INS-1 cells with PSCs-CM at 25, 50 and 75% (v/v) caused a significant and concentration dependent reduction in cell number compared to control INS-1 cells incubated in standard tissue culture medium. The deleterious effects of PSCs-CM were significant by 24 h, and were further enhanced after 48 h treatment (a). (b) shows that the treatment with PSCs-CM or HG for 24 h were alone sufficient to reduce INS-1 cell viability, and that their effects were additive, with the combination of PSCs-CM and HG exerting a significantly more detrimental effect than either treatment alone. All data were expressed as mean ± SE (𝑛=9), ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control.

(a)

(b)